Article Archive

 
 

 

 

 

 

 

Volume 1, Issue 2

 

 

Physiological Correlation between Sperm Motility and Seminal Plasma Composition in the Persian Sturgeon, Acipenser persicus

Journal of Infertility and reproductive Biology, 2013, Volume 1, Issue 2, Pages 12-20, Full Text in PDF (206 KB)

Mohammad Sadegh Aramli1, Mohammad Reza Kalbassi1, Rajab Mohammad Nazari2

1. Aquaculture Department, Tarbiat Modares University, Noor, Mazandaran, Iran

2. Rajaee Sturgeon Hatchery Center, Sari, P.O. Box 833, Sari, Mazandaran, Iran

 

Abstract

Knowledge of the reproductive physiology of wild sturgeon populations is critical to ensure the survival of this unique group of animals. This experiment was designed to explore the physiological relationships between the seminal plasma composition (ionic and organic contents as well as osmolality) and sperm motility in the Persian sturgeon (Acipenser persicus). In this regard, 17 brood fish were injected intramuscularly by LHRH-A2 (5μgKg -1) for spermiation induction. According our results, the seminal plasma contained 59.53±2.56 mM/l sodium, 9.1±1.42 mM/l chloride, 4.72±0.3 mM/l potassium, 1.45±0.075 mM/l calcium and 0.7±0.072 mM/l magnesium. 0.11±0.02 g/dl total protein, 22.18±4.16 mg/dl glucose, 6.67±1.04 mg/dl cholesterol and 15.2±0.65 mg/dl triglyceride. The total duration of sperm movement was obtained 288.01±14.44 second. Also, the osmolality of seminal plasma ranged from 47 to 176 mOsmol kg1. Significant positive relationships were determined between sperm motility vs. K+ ion (r=0.718, p<0.01) and total protein (r=0.670, p<0.01). No significant correlations were found between sperm motility and others composition of the seminal plasma. Presented data could be considered as a complementary study for developing special extenders and protectant solutions for improving artificial fertilization in this valuable species.

 

Keywords: Spermatozoa motility, seminal plasma composition, Acipenser persicus.

 

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Alpha Deposit into Blood: A New Method to Evaluate Infertility of Women by Measuring the Level of LH, FSH and HCG

 

Journal of Infertility and reproductive Biology, 2013, Volume 1, Issue 2, Pages 21-25, Full Text in PDF (99 KB)

 

Najeba F. Salih1, Mohamad S. Jaafar1

 

Medical Physics and Radiation Science Research Group, School of Physics, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.

 

Abstract

This study examines the effect of alpha particles radioactive exposure on pregnancy hormones such as luteinizing hormones (LH), follicle stimulating hormone (FSH), hormone chorionic gonadotropin (HCG), progesterone and estradiol, in order to investigate the prevalence of infertility. Done Hormonal analysis of the women blood samples and irradiated by radium source after then carried out the hormone testing. This disparity supports the hypothesis that the magnitude of changes in pregnancy hormones concentration is indicative of the effect of radiation on the human tissues. To ascertain the level of ovarian reserve, the FSH level was measured, since it correlates inversely with the number of eggs present in ovaries. High levels of FSH were observed in women with low ovarian reserve. Significant differences were found before and after radiation. For LH (p<0.001), HCG (p<0.001), and estradiol (p<0.001), the levels of these hormones significantly reduced after radiation, while elevation observed for progesterone (p<0.003), FSH (p<0.003). This study concluded that FSH is significantly increased by irradiation which subsequently leads to impaired fertility.

 

Keywords: FSH, LH, pregnancy hormones concentration, Estradiol, women infertility, Alpha particles

 

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Restraint Stress and Lead-Induced Reduction in Progeny Output by Male Rats

 

Journal of Infertility and reproductive Biology, 2013, Volume 1, Issue 2, Pages 26-30, Full Text in PDF (104 KB)

 

P. Hari Priya1, B.P. Girish1,2, P. Sreenivasula Reddy2

 

1. Department of Biotechnology, Sri Venkateswara University, Tirupati – 517502, India

2. Department of Zoology, Sri Venkateswara University, Tirupati – 517502, India

 

Abstract

We investigated whether restraint stress interferes with lead-induced suppressed fertility in rats. Male Wister rats (weighing 200 ± 10 g in the beginning of the experiment) were subjected to either restraint stress (5 hours/day) or exposed to lead (0.15%) or both for 60 days. To assess the fertility, control and experimental males were cohabited with sexually mature normal females. No significant changes (p>0.05) were observed in the body weight gain of experimental males. Similarly, no changes (p>0.05) were observed in the mating index. In contrast, there was a significant decrease (p<0.05) in fertility rate, measured by counting live foetuses in the uterus of normal females mated with males exposed to either restraint stress or lead. In rats mated to males subjected to both restraint stress and lead treatment, a significant decrease (p<0.05) in live fetuses was observed as compared to rats mated with lead alone exposed males.These data demonstrate that adult male rats exposed to either restraint stress or lead significantly decreased fertilization capacity and stress potentiates the reproductive toxicity of lead.

Keywords: Lead, Restraint stress, Fertility, Implantation loss, Rat.

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Etiology, Investigation and Treatment of Human Men’s Infertility

 

Journal of Infertility and reproductive Biology, 2013, Volume 1, Issue 2, Pages 31-36, Full Text in PDF (83 KB)

 

J.Poongothai

 

Assistant Professor in Biotechnology, PSG College of Technology, Coimbatore,Tamil Nadu, India.

 

Abstract

Infertility is a worldwide reproductive health and emotionally charged problem that affects approximately 15% of married couples. In recent years, male infertility has increased in the industrialized countries due to a decline in sperm counts and a rise in testicular and sperm pathologies. There seems to be a direct relationship between modernlifestyles and declining male fertility and the statistics are alarming. In this review, etiological factors of human male infertility known up to date and the techniques employed in fertility research are evaluated.

 

Keywords: Infertility, subfertile, etiology Regards, J.Poongothai

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Association Between Maternal Serum Inhibin A and Chromosomal Abnormalities as a Diagnostic Markers in First Trimester of Threatened Abortion

 

Journal of Infertility and reproductive Biology, 2013, Volume 1, Issue 2, Pages 37-42, Full Text in PDF (1210 KB)

 

Mohamed Gamal1, Neveen A. Ashaat2, Sayed Bakry1, Ahmed Abdullah3, Mohamed Farahat4, Zaki T. Zaki1

 

1. Zoology Department, school of Scienc, Al Azhar University, Cairo, 11884, Egypt.

2. Zoology Department, Faculty of Women for Arts, Science & Education, Ain Shams University, Cairo, Egypt.

3. Biochemistry Department, school of Pharmacy, Al Azhar University, Cairo, Egypt.

4. Obstetrics & Gynecology Department, School of Medicine, Al Azhar University, Cairo, Egypt.

 

Abstract

Background: Threatened abortion is defined as a pregnancy complicated by bleeding before 20 weeks’ gestation. Objective: Assessment of the relation between the concentration of serum Inhibin A and chromosomal abnormalities in a trial to find marker for the threatened abortion. Patients and Methods: The present study included 40 pregnant women in the first-trimester, 20 pregnant women suffering from pregnancy complications (threatened aborted) and 20 pregnant women for control. Blood samples were collected from El Hussain Hospital, Cairo, Egypt, under the clinical supervision. Serum is prepared for measurement Inhibin A hormone using Enzyme-Linked Immuno-Sorbent Assay (ELISA), while heparinized blood processed for cell culture to determine chromosomal aberrations. Results: Cytogenetic analysis revealed that structural chromosomal abnormalities were recorded in 35 from 600 maternal metaphase (5.83%), compared to (2.6%) that were detected in the examined metaphases of control group; there was no statistical significance between the control and threatened aborted cases (χ˛ = 0.85, P ˃ 0.05), while concentration of Inhibin A hormone in the studied group shows Mean of 16.7 pg/ml but in control group shows concentration of 38.7 pg/ml, there was a significant difference between the two groups in serum level of inhibin A (t = 12.9, P < 0.05). On the other hand we found no correlation between chromosomal anomalies and inhibin A level in our cases (r = -0.296, p=0.204). Conclusion: Serum inhibin A measured during the first trimester of pregnancy might be useful for prediction of threatened abortion.

 

Keywords: Threatened abortion, Inhibin A, Chromosomal abnormalities.

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