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Effect of fenugreek seed extract on carbofuran-inhibited spermatogenesis and induced
apoptosis in albino rats
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 2, Pages 36-42,
Full
Text in PDF (423 KB)
Sakr SA, Shalaby SY
Department of Zoology,
Faculty of Science, Menoufia University, Shebin El-kom, Egypt
Abstract
Carbofuran is a broad spectrum carbamate pesticide
used against different pests. Fenugreek (Trigonella foenum graecum)
is used as a medicinal plant and showed many therapeutic effects. The present work studied the effect of aqueous extract of fenugreek
seeds on carbofuran induced testicular toxicity in albino rats. Treating rats with carbofuranfor 6 weeks induced
significant decrease in testis weights,
diameters and germinal epithelial heights of the seminiferous tubules.
Histological results revealed
intertubular hemorrhage, degeneration of the interstitial tissue and
reduction of spermatogenic cells. Expression of caspase-3 and bax increased
in the vgerm cells. Biochemical results showed decrease of testosterone and
LH in sera of the treated animalsCoadministration of carbofuran with fenugreek
seeds extracts ameliorates the histopathological alterations caused by
carbofuran in testes of albino rats. Moreover, it caused decrease of
apoptosis as indicated by decrease of expression of caspase-3 and bax, and
increased testosterone and LH.It is concluded that the
effect of fenugreek against testicular toxicity of carbofuran may be due to the antioxidant activity of
its constituents (e.g. flavonoids and polyphenols).
Keywords:
Carbofuran, Fenugreek, Testis, Rat, Caspase-3, Antioxidants
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Relevance of serum ascorbic acid status in ovulation and pregnancy
outcome of non-PCOS women undergoing intrauterine insemination cycles
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 2, Pages 43-49,
Full
Text in PDF (96 KB)
Shaikh N1,
Shinde G.B1, Nath N2
1. Postgraduate Teaching
Department of Biochemistry, RTM Nagpur University, Nagpur, Maharashtra,
India
2. Department of
Biochemistry Research Laboratory, Vaunshdhara Clinic and Assisted
Conception Centre, Nagpur, Maharashtra, India
Abstract
Ovulation is an important
process for successful conception in intra-uterine insemination (IUI)
cycles. The ovulatory process is initiated by an E2 induced LH surge causes
a series of dramatic physiological andbiochemical alterations in the ovary
leading to follicle rupture and oocyte release. Ascorbic acid (AA) has
implicated in the process of ovulation and folliculogenesis. Also
itsaltered levels have been correlated to luteal-steroidogenesis. Hence,
the study attempts to carry out an inter-phasic comparison of various
hormones and AA between the early follicular, periovulatory and
luteal-phases in non-PCOS women during IUI-cycle so as to obtain a cogent
view about their implications in ovulatory process and subsequent
pregnancy-outcome. AA level is found to be at its nadir in the ovulatory
phase while LH level is at its zenith. The utilization of AA in the
ovulatory phase is seen to hold the key for future course of events i.e.
synthesis of 17-OHP and E2, leading to pregnancy. A significant decrease
was observed in the serum-AA levels from follicular to ovulatory phase within the pregnant group
but not in the non-pregnant ones. Thus, serum ascorbic-acid seems to have a
prominent bearing for ovulation and pregnancyoutcome
in non-PCOS women undergoing IUI-cycles.
Keywords:
Ascorbic acid, Ovulation, IUI, Pregnancy
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Physiological and molecular determinants of embryo-uterine
interactions in ruminants
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 2, Pages 50-55,
Full
Text in PDF (95 KB)
Tripathi
SK1, Farman M2, Nandi S1, Gupta PSP1,
Girish Kumar V2
1. National Institute of
Animal Nutrition and Physiology (NIANP), Bangalore, India
2. College of Veterinary
Sciences, KVAFSU, Bangalore campus, Hebbal, Bangalore, India
Abstract
Maternal recognition of
pregnancy (MRP) manifests
the various ways
in which the
mother responds to
the presence of a conceptus within
her reproductive tract.
Corpus luteum produces
progesterone, the hormone
of pregnancy, which is required to stimulate and maintain
endometrial functions that are permissive to early embryonic development,
implantation, placentation, and successful fetal and placental development.
The interaction between a competent embryo and a receptive uterine
environment is responsible for successful growth and development of the post-hatching
blastocyst and pregnancy establishment. Certain hormones, enzymes,
cytokines, interleukins and gene transcripts contribute and
regulate the bidirectional channel
of communication during
the pregnancy period
in ruminants. During Maternal recognition of
pregnancy some genes like
interferon-tau (IFNT), Ubiquitin
Cross Reactive Protein (UCRP),
Ghrelin,
Aldoketoreductase-1B5
(AKR1B5), SERPINA14 are
appear to have
role insuccessful
establishment of pregnancy and expression of the cascade of signaling
molecules. These genes regulate the endometrial environment to establish
pregnancy in farm animals. MRP in
ruminants requires that theconceptus elongates from
a spherical to
a tubular and
then filamentous form
to produce IFNT
which is the
pregnancy recognition signal. Genetic factors of both embryo and mother’s endometrium are also
responsible for successful embryo development.
Keywords:
Maternal recognition of pregnancy, Corpus luteum, Gene, Conceptus
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The status of
ascorbic acid in follicular fluid of non-PCOS women during IVF-ET cycles is
an indicator for aromatization andpregnancy outcome
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 2, Pages 56-61, Full
Text in PDF (96 KB)
Shaikh N1,
Shinde G.B1, Nath N2
1. Postgraduate Teaching
Department of Biochemistry, RTM Nagpur University, Nagpur, Maharashtra,
India
2. Department of
Biochemistry Research Laboratory, Vaunshdhara Clinic and Assisted
Conception Centre, Nagpur, Maharashtra, India
Abstract
Follicular fluid (FF)
serves as a natural ‘culture medium’ for the maturation of both granulosa
cells and oocytes. Ascorbic Acid (AA) known
to maintain reproductive integrity and reported to either accumulate
in the follicular fluid or other ovarian cells. A relatively higher bioavailability of AA
within the graafian follicle as compared to serum suggests importance of AA
in the graafian follicle biology. As a pre-eminent water soluble
antioxidant, this molecule has also
been reported to
provide protection of
oocyte and granulosa
cells against cellular
injuries but earlier studies
were limited with
exogenous
supplementation. The present
study evaluated the
significance of endogenous FF-ascorbic acid in aromatization and pregnancy outcome in
non-PCOS women undergoing IVF-ET treatment cycles. The positive
correlationship of FF-AA with
clinical pregnancy rate observed in thisstudy clearly denotes the
importance of maintaining the
antioxidant status within
the microenvironment in
order to achieve pregnancy. The
present study has
attempted to establish
for the first
time a successful correlationship between endogenous FF-AA levels with
pregnancy outcome as against the exogenous supplementation theory and also
aided to determine the critical requirement of AA within the follicular
milieu for achieving the objective.
Keywords:
Ascorbic acid, DHEA-S, E2, Aromatization, Pregnancy
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Cadmium and male infertility
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 2, Pages 62-69,
Full
Text in PDF (108 KB)
Alaee S1,
Talaiekhozani A2,3, Rezaei S4, Alaee K5,
Yousefian E6
1. Department of
Reproductive Biology, School of Advanced Medical Sciences and Technologies,
Shiraz
University of Medical
Sciences, Shiraz, Iran
2. Institute
of Environmental and
Water Resources Management, Water
Research Alliance, Universiti
Teknologi Malaysia, UTM
Skudai, 81310 Johor Bahru, Malaysia
3. Jami Institute of
Technology, Department of Civil Engineering, Isfahan, Iran
4. Department of
Obstetrics and Gynecology, Shiraz University of Medical Sciences, Shiraz, Iran
5. Department of
Radiology, Shiraz University of Medical Sciences, Shiraz, Iran
6. Department of
Midwifery, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Abstract
Cadmium (Cd)
is a heavy
metal to which
humans are exposed
both occupationally and
environmentally. For many
years cadmium has been understood as a toxic element to human health, and
an elevated level of cadmium exposure has been shown to be related to
adverse reproductive effects, especially in men. In this review we studied
published data about the toxic effects of this trace element on the total
male reproductive system, including gonadal development, testes,
testosterone, spermatogenesis and
accessory sex glands, to clarify how cadmium causes male fertility problems.
For this purpose,
in the next
sections after introducing this
trace element thoroughly, we
will separately mention cadmium’s effects on each part of male
reproductive system.
Keywords:
Cadmium, Male, Testis, Sperm, Reproduction
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