Article Archive

 

 

 

 

 

 

 

 

Volume 2, Issue 2

 

 

 

Effect of fenugreek seed extract on carbofuran-inhibited spermatogenesis and induced apoptosis in albino rats

 

Journal of Infertility and reproductive Biology, 2014, Volume 2, Issue 2, Pages 36-42, Full Text in PDF (423 KB)

 

Sakr SA, Shalaby SY

 

Department of Zoology, Faculty of Science, Menoufia University, Shebin El-kom, Egypt

 

Abstract

Carbofuranis a broad spectrum carbamate pesticide used against different pests. Fenugreek (Trigonella foenum graecum) is used as a medicinal plant and showed many therapeutic effects. The present work studied the effect of aqueous extract of fenugreek seeds on carbofuran induced testicular toxicity in albino rats. Treating rats with carbofuranfor 6 weeks induced significant decrease in testis weights, diameters and germinal epithelial heights of the seminiferous tubules. Histological results revealed intertubular hemorrhage, degeneration of the interstitial tissue and reduction of spermatogenic cells. Expression of caspase-3 and bax increased in the vgerm cells. Biochemical results showed decrease of testosterone and LH in sera of the treated animalsCoadministration of carbofuran with fenugreek seeds extracts ameliorates the histopathological alterations caused by carbofuran in testes of albino rats. Moreover, it caused decrease of apoptosis as indicated by decrease of expression of caspase-3 and bax, and increased testosterone and LH.It is concluded that the effect of fenugreek against testicular toxicity of carbofuran may be due to the antioxidant activity of its constituents (e.g. flavonoids and polyphenols).

 

 

Keywords: Carbofuran, Fenugreek, Testis, Rat, Caspase-3, Antioxidants

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Relevance of serum ascorbic acid status in ovulation and pregnancy outcome of non-PCOS women undergoing intrauterine insemination cycles

 

Journal of Infertility and reproductive Biology, 2014, Volume 2, Issue 2, Pages 43-49, Full Text in PDF (96 KB)

 

Shaikh N1, Shinde G.B1, Nath N2

 

1. Postgraduate Teaching Department of Biochemistry, RTM Nagpur University, Nagpur, Maharashtra, India

2. Department of Biochemistry Research Laboratory, Vaunshdhara Clinic and Assisted Conception Centre, Nagpur, Maharashtra, India

 

Abstract

Ovulation is an important process for successful conception in intra-uterine insemination (IUI) cycles. The ovulatory process is initiated by an E2 induced LH surge causes a series of dramatic physiological andbiochemical alterations in the ovary leading to follicle rupture and oocyte release. Ascorbic acid (AA) has implicated in the process of ovulation and folliculogenesis. Also itsaltered levels have been correlated to luteal-steroidogenesis. Hence, the study attempts to carry out an inter-phasic comparison of various hormones and AA between the early follicular, periovulatory and luteal-phases in non-PCOS women during IUI-cycle so as to obtain a cogent view about their implications in ovulatory process and subsequent pregnancy-outcome. AA level is found to be at its nadir in the ovulatory phase while LH level is at its zenith. The utilization of AA in the ovulatory phase is seen to hold the key for future course of events i.e. synthesis of 17-OHP and E2, leading to pregnancy. A significant decrease was observed in the serum-AA levels from follicular toovulatory phase within the pregnant group but not in the non-pregnant ones. Thus, serum ascorbic-acid seems to have a prominent bearing for ovulation and pregnancyoutcome

in non-PCOS women undergoing IUI-cycles.

 

Keywords: Ascorbic acid, Ovulation, IUI, Pregnancy

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Physiological and molecular determinants of embryo-uterine interactions in ruminants

 

Journal of Infertility and reproductive Biology, 2014, Volume 2, Issue 2, Pages 50-55, Full Text in PDF (95 KB)

 

Tripathi SK1, Farman M2, Nandi S1, Gupta PSP1, Girish Kumar V2

 

1. National Institute of Animal Nutrition and Physiology (NIANP), Bangalore, India

2. College of Veterinary Sciences, KVAFSU, Bangalore campus, Hebbal, Bangalore, India

 

Abstract

Maternalrecognitionofpregnancy(MRP)manifeststhevariouswaysinwhichthemotherrespondstothe presenceofa conceptuswithinherreproductivetract.Corpusluteumproducesprogesterone,thehormoneof pregnancy, which is required to stimulate and maintain endometrial functions that are permissive to early embryonic development, implantation, placentation, and successful fetal and placental development. The interaction between a competent embryo and a receptive uterine environment is responsible for successful growth and development of the post-hatching blastocyst and pregnancy establishment. Certain hormones, enzymes, cytokines, interleukins and gene transcriptscontributeandregulatethebidirectionalchannelofcommunicationduringthepregnancyperiodin ruminants.During Maternalrecognitionofpregnancy somegeneslikeinterferon-tau(IFNT),UbiquitinCross ReactiveProtein(UCRP),Ghrelin,Aldoketoreductase-1B5(AKR1B5),SERPINA14areappeartohaveroleinsuccessful establishment of pregnancy and expression of the cascade of signaling molecules. These genes regulate the endometrial environment to establish pregnancyin farm animals. MRP in ruminants requires that theconceptus elongatesfromasphericaltoatubularandthenfilamentousformtoproduceIFNTwhichisthepregnancy recognition signal. Genetic factors of both embryoand motherís endometrium are also responsible for successful embryo development.

 

Keywords: Maternal recognition of pregnancy, Corpus luteum, Gene, Conceptus

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The status of ascorbic acid in follicular fluid of non-PCOS women during IVF-ET cycles is an indicator for aromatization andpregnancy outcome

 

Journal of Infertility and reproductive Biology, 2014, Volume 2, Issue 2, Pages 56-61, Full Text in PDF (96 KB)

 

Shaikh N1, Shinde G.B1, Nath N2

 

1. Postgraduate Teaching Department of Biochemistry, RTM Nagpur University, Nagpur, Maharashtra, India

2. Department of Biochemistry Research Laboratory, Vaunshdhara Clinic and Assisted Conception Centre, Nagpur, Maharashtra, India

 

Abstract

Follicular fluid (FF) serves as a natural Ďculture mediumí for the maturation of both granulosa cells and oocytes. Ascorbic Acid (AA) knownto maintain reproductive integrity and reported to either accumulate in the follicular fluid or other ovarian cells. Arelatively higher bioavailability of AA within the graafian follicle as compared to serum suggests importance of AA in the graafian follicle biology. As a pre-eminent water soluble antioxidant, this moleculehasalsobeenreportedtoprovideprotectionofoocyteandgranulosacellsagainstcellularinjuriesbut earlierstudieswerelimitedwithexogenoussupplementation.Thepresentstudyevaluatedthesignificanceof endogenousFF-ascorbic acidin aromatization and pregnancy outcome in non-PCOS women undergoing IVF-ET treatment cycles. The positive correlationship ofFF-AA with clinical pregnancy rate observed in thisstudy clearly denotestheimportanceofmaintainingtheantioxidantstatuswithinthemicroenvironmentinordertoachieve pregnancy.Thepresentstudyhasattemptedtoestablishforthefirsttimeasuccessfulcorrelationshipbetween endogenous FF-AA levels with pregnancy outcome as against the exogenous supplementation theory and also aided to determine the critical requirement of AA within the follicular milieu for achieving the objective. 

 

Keywords: Ascorbic acid, DHEA-S, E2, Aromatization, Pregnancy

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Cadmium and male infertility

 

Journal of Infertility and reproductive Biology, 2014, Volume 2, Issue 2, Pages 62-69, Full Text in PDF (108 KB)

 

Alaee S1, Talaiekhozani A2,3, Rezaei S4, Alaee K5, Yousefian E6

 

1. Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz

University of Medical Sciences, Shiraz, Iran

2.InstituteofEnvironmentalandWaterResourcesManagement,WaterResearchAlliance,Universiti

Teknologi Malaysia, UTM Skudai, 81310 Johor Bahru, Malaysia

3. Jami Institute of Technology, Department of Civil Engineering, Isfahan, Iran

4. Department of Obstetrics and Gynecology, Shiraz University of Medical Sciences, Shiraz, Iran

5. Department of Radiology, Shiraz University of Medical Sciences, Shiraz, Iran

6. Department of Midwifery, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

 

Abstract

Cadmium(Cd)isaheavymetaltowhichhumansareexposedbothoccupationallyandenvironmentally.For many years cadmium has been understood as a toxic element to human health, and an elevated level of cadmium exposure has been shown to be related to adverse reproductive effects, especially in men. In this review we studied published data about the toxic effects of this trace element on the total male reproductive system, including gonadal development, testes, testosterone, spermatogenesisand accessory sex glands, to clarify how cadmium causes male fertilityproblems.Forthispurpose,inthenextsectionsafterintroducingthistraceelementthoroughly,wewill separately mention cadmiumís effects on each part of male reproductive system. 

 

Keywords: Cadmium, Male, Testis, Sperm, Reproduction


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