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The Expression of β-Catenin in the
Epithelial Cells and Stromal Cells of
Endometriosis and normal endometrial cells
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 3, Pages 70-76,
Full
Text in PDF (174 KB)
Kanza MR
Department
of obstetrics and gynecology of union hospital tongji
medical college, wuhan-hubei province, China
Abstract
Endometriosis is a common
benign disease defined by the presence of the endometrial epithelial and stromal cells outside of the uterine cavity. To date, the etiology, pathogenesis and
path-physiology are not fully clarified. The understanding of the cellular
and molecular mechanisms of pathogenesis of endometriosis is crucial in
novel strategic therapeutic approaches for the endometriosis. The purpose
of the present study was to examine the expression pattern,
the intensity and the sub localization of β-catenin
in both ectopic and eutopic endometrial cells
during the proliferative phase of the menstrual cycle. A total number of 41
tissue samples were included in this study, where endometriotic
tissue samples (n=26）and normal endometrial tissue samples (n=15) were
investigated using immunohistochemistry（IHC）method to detect the expression patterns
of β-catenin. While, the Immunoperoxidase
staining technique and semi-quantitative scoring system were used to
quantify the results and the comparisons between endometriotic
tissues and normal endometrial tissues were also performed. Compared with
normal endometrial tissues, our study found a decreased β-catenin
expression in endometriotic tissues. Both, Endometriotic tissues of the ovary from non-pregnant
and the peritoneum showed a statistically significant reduced- β-catenin expression
(P <0.001) when compared with the normal endometrial cell (P < 0.
05). Besides the Different expression modes of β-catenin in ectopic and eutopic endometrial cells was also been detected. In
conclusion the down-expression of β-catenin may have an essential role in the invasion,
proliferation and differentiation of endometriosis. The alteration of
expression of β-catenin may take part in the pathogenesis of
endometriosis.
Keywords: β-Catenin, Endometriosis, Normal endometrial cells,
Epithelial cells, Stromal cells
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FKBP51 and FKBP52 as potential biomarkers for predicting endometrial
receptivity and embryo implantation in assisted reproductive technologies
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 3, Pages 77-81,
Full
Text in PDF (72 KB)
Alaee S1, Ghaffari Novin M2,3, Yeganeh
F4
1. Department of
Reproductive Biology, School of Advanced Medical Sciences and Technologies,
Shiraz University of Medical Sciences, Shiraz, Iran
2. Department of Biology and
Anatomical Sciences, Faculty of medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3. Infertility and Reproductive Health Research
Center (IRHRC), Shahid Beheshti
University of Medical Sciences, Tehran, Iran.
4.
Department of Immunology, Faculty of Medicine, Shahid
Beheshti University of Medical Sciences, Tehran,
Iran
Abstract
One of the most important and limiting
factors in assisted reproductive technologies (ART) is repeated
implantation failure (RIF). In the process of embryo implantation,
appropriate function of the progesterone hormone through its receptors is
critical for establishment of a receptive endometrium.
FKBP51 and FKBP52 are two co-chaperones of progesterone receptors that
participate in the progesterone signaling pathway and endometrial
receptivity. These 51 and 52 KD proteins increase and decrease the affinity
of progesterone hormone receptors to their ligand,
respectively. The endometrial tissue of RIF
patients might have different patterns of FKBP51 and FKBP52 gene
expression. These molecules can easily be detected and quantified by
quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and immunohistochemical
(IHC) studies. Assessment of these molecules could be a valuable method for
evaluation of endometrial receptivity and prediction of the implantation
and pregnancy outcome before beginning a treatment cycle for RIF patients in ART procedures.
Keywords: ART, Repeated implantation failure, Progesterone,
FKBP51, FKBP52
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Nonylphenol exposure disrupts the fertility efficiency in
adult male rats
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 3, Pages 82-87,
Full
Text in PDF (768 KB)
U. Kanchana
ganga1, B. Kishori1, P. Sreenivasula
Reddy2
1. Department of
Biotechnology, Sri Padmavati Mahila
Visva Vidyalayam, Tirupati, A.P., India
2. Department of Zoology,
Sri Venkateswara University, Tirupati,
A.P., India
Abstract
The present
research was aimed
to investigate the
effects of low
doses of nonylphenol on
male reproductive performance
in adult rats. Thirty two male Wistar rats were
randomly divided into four groups (weighing 160 ± 10 g in the beginning of
the experiment). First group served as control and were injected with only
DMSO, remaining second, third and fourth groups were administered with nonylphenol of 1, 10, 100 µg/kg Bw
through intr aperitonial
injections for 55 days. To observe the fertility, control and experimental
males were cohabited with sexually mature normal females. A significant (p<0.05) decrease
was observed in the body weight
gain of experimental males.
Significant (p<0.05) changes were not observed in the mating index.
There was a significant (p<0.05) decrease in fertility rate,
measured by counting
live fetuses in
the uterus of
normal females mated
with males exposed
to nonylphenol. Four female rats were
sacrificed on 8thday of pregnancy, ovaries were
examined for the numbers of corpora lutea.
Remaining four
female rats were sacrificed on 18thday of pregnancy and uteri
were dissected and examined for determination of numbers of implantations,
dead and live fetuses, and resorptions. Females
mated with 100 µg/kg Bw nonylphenol
exposed males showed resor ptions
and a significant (p<0.05) decrease in number of live fetuses per rat.
The present results revealed significant decrease in fertilization capacity
of adult male rats exposed to nonylphenol.
Keywords: Xenoestrogens, Nonylphenol, Fertility, Implantation loss, Rat
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Serum gonadotropin and prolactin
levels in females with primary infertility and thyroid dysfunction in North
Indian population
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 3, Pages 88-91,
Full
Text in PDF (61 KB)
A. Yadav1, M.
Arora2, V. Saini2, J. Bhattacharjee3, A.
Jain3
1. Department of
Biochemistry, Lady Hardinge Medical College, New
Delhi, India
2. Lady Hardinge Medical College, New Delhi, India
3. Lady Hardinge Medical College, New Delhi, India
Abstract
Adequate levels of
circulating thyroid hormones
are of primary importance for
normal reproductive function. Patients with subclinical
hypothyroidism have no symptoms or very few symptoms related to thyroid
disease, but may have adverse outcome on fertility but are not referred to
infertility clinics. Galactorrhea and hyperprolactinemia patients may have primary
hypothyroidism. The aim of this study was to evaluate if thyroid dysfunctionleads to any alteration in serum gonadotropin and prolactin
levels contributing towards infertility.
This case control study was performed in 256 females in a tertiary care hospital.Serum TSH, fT4 and fT3, LH, FSH and prolactin levels were assayed using fully automated chemiluminescent immunoassay Analyzer Access 2 by
Beckman and Coulter (USA). Serum LH, FSH levels were significantly low and
serum prolactin levels were significantly high in
patients with infertility
and
hypothyroidism. Serum LH,
FSH levels were
significantly high in
infertile females with hyperthyroidism whereas serum prolactin levels werelow but
not significantly different in these patients. Thyroid disorders have a
great impact on fertility in females. Fertility improves when euthyroidism is restored. It has been suggested that
normal gonadotropin and thyroid function tests
are necessary to achieve maximum fertilization rate and blastocyst
development. Thyrotoxicosis in womenhas been linked with reduced fertility but the
mechanism for the same has not been elucidated clearly. We suggest
screening of thyroid hormone in all the females with infertility.
Keywords: Gonadotropin, Prolactin, Primary infertility, Thyroid dysfunction
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Every month becomes a funeral when they menstruate: African women’s
beliefs about couple infertility
Journal of Infertility and reproductive Biology, 2014,
Volume 2, Issue 3, Pages92-100,
Full
Text in PDF (113 KB)
F. Naab
Department of Maternal and
Child Health ,College of Health Sciences, School of Nursing ,University of
Ghana
Abstract
The experience of infertility in Africa is
culturally constructed. However, the influence of these cultural beliefs
about infertility on the experience of infertility in Africa is yet to
receive scientific exploration. The purpose of this study was to explore
beliefs about couple infertility among women from different African
cultures. Fifteen African women volunteers from eight African countries
were recruited from an African Women Association in a northern state of the
United States of America for this qualitative study. Individual
in-depth semi-structured interviews were conducted between June and
September, 2008. Each interview lasted 30 to 45 minutes. Questions asked
included beliefs about couple infertility in their respective cultures. All
interviews were audio taped and transcribed verbatim. Data were
content-analyzed and Nvivo 8 was used to manage and organize the data.
Cultural expectation emerged as a main theme, within which originated three
areas of beliefs; community attitudes, family influences, couples’
reactions to infertility and coping strategies. The findings showed that
beliefs about couple infertility in the eight African countries were
similar. The findings suggested a strong belief that culturally, African
men do not become infertile. As a result, women are blamed for couple
infertility. The findings further showed that women with couple infertility
are vulnerable to sexually transmitted infections such as HIV/AIDS. It is
important to include sexual health counseling in the management of couples
with infertility in Africa.
Keywords: Beliefs,
Infertility, Couple infertility, African Women
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