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J. Infertil. Reprod. Biol.

Volume 2, Issue 4, 2014

 

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Altered male reproduction in rats exposed perinatally to biochanin-A

 

Journal of Infertility and reproductive Biology, 2014, Volume 2, Issue 4, Pages 101-107, | Full Text in PDF (361 KB)

 

M. G. S. Soujanya1, K. Pratap Redddy2, P. Ramachandra Reddy1, P. Sreenivasula Reddy2

 

1. Department of Biochemistry, Yogi Vemana University, Vemanapuram, Kadapa – 516 003, A.P. India

2. Department of Zoology, Sri Venkateswara University, Tirupati – 516 003, A.P. India

 

Abstract

Phytoestrogens are the plant extract compounds, primarily isoflavones that mimic or modulate endogenous estrogens, usually by binding to estrogen receptors. Biochanin- A (BC-A) is an isoflavone found in red clover and is a selective agonist of estrogen receptor-β (ER-β) and have the capacity to alter functioning of male reproduction. The present study focused on the changes of reproduction in male rats exposed to BC-A during the perinatal period. Normal adult male and female rats were co-habitated and the pregnant female rats were injected with 25mg, 50mg and 100 mg BC-A/Kg body weight on window period 12th, 14th, 16th and 18th day.  Rats were allowed to deliver the pups and the BC-A exposure was continued up to weaning period. After weaning male and female pups were separated and maintained in separate cages in a dose wise manner. To check the reproductive performance of adult males (90 days old) exposed perinatally to BC-A are co-habited with sexually mature normal females. Female rats were sacrificed by cervical dis-location and autopsy was performed on 8th and 19th day of gestation. Uterus and ovaries were examined for the numbers of corpora lutea, implantations, dead, live foetuses, resorptions and fetal weight. Significant increase was noticed in the conception time, pre and post implantation loss (p<0.0001) and decrease in the number of implantations (p<0.0001). Further observed significant decrease in fertility rate as measured by counting numbers of live fetuses (p<0.005) and resorptions (p<0.05) in the females mated with males exposed to BC-A. Females mated with BC-A exposed males were shown significant reduction (p<0.0001) in resorptions, and significant decrease (p<0.05) in fetal weight and number of live fetuses per rat. The present study elucidated the significance of perinatal BC-A exposure on male reproductive health.

 

Keywords: Biochanin-A, Isoflavonoid, Fertility rate, Implantation loss, Testosterone, Male wistar rats

 

 

Correlation between women age and oocyte quality, embryo formation and pregnancy outcomes in assisted reproductive technology (ART) cycles: a retrospective analysis

 

Journal of Infertility and reproductive Biology, 2014, Volume 2, Issue 4, Pages 108-114, |  Full Text in PDF (99 KB)

 

M H. Razi1 , Y. Razi2 , P .Sabeti1 , S. Ghasemi-Esmailabad1 , S. Pourmasumi1

 

1-Recearch and Clinical Center for Infertility, Shahid Sadughi University of Medical Sciences, Yazd, Iran

2-International Campus of Shahid Sadoughi University of Medical Sciences, Yazd, Iran

 

Abstract

It is documented that there is a decline in female fertility as a function of age. To estimate the effect of women age on assisted reproductive technology (ART) outcomes, a total of 1560 in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles were initiated in different age-groups women. (Group 1: age ≤ 25, Group 2: age between 26-30 , Group 3: age between 31-35, Group 4: age between 36-40, Group 5: age > 40). Patient’s data and pregnancy outcomes were ascertained by records review and face-to-face follow-up. Age, number of retrieved oocytes and matured oocytes, types of ART procedures, number of fertilized oocytes, zygote and embryo scores, as well as, pregnancy and delivery rates were evaluated. The results showed a significantly negative correlation between age of patients and the number of oocytes retrieved (P<0.01), mature oocytes (P<0.001), and fertilized oocytes (P<0.01) in five age- groups. We found no significantly differences in the number of mature oocytes in the 26–30 age group with the women who aged between 31 and 35 (P=0.066). The zygote and embryo scores only showed a significant negative correlation with age in women more than 40 years of age (P≤ 0.01 for each). However, a statistically negative correlation was found between the age of women with clinical pregnancy and delivery rates (P=0.031 and P=0.006, respectively). No striking differences found in pregnancy rate between IVF and ICSI procedures (P=0.095 and P=0.117, respectively). Our findings recommend that the age-related decline in female fertility by effect on oocyte and embryo quality. The women age negatively influence outcome of assisted reproductive technology treatment.

 

Keywords: Women age, Pregnancy rate, Intracytoplasmic sperm injection, In vitro fertilization

 

 

Perinatal exposure of biochanin-A induced abnormalities in offspring of rats

 

Journal of Infertility and reproductive Biology, 2014, Volume 2, Issue 4, Pages 115-123, |  Full Text in PDF (1.26 MB)

 

M. G. S. Soujanya1, P. Ramachandra Reddy1, P. Sreenivasula Reddy2

 

1. Department of Biochemistry, Yogi Vemana University, Vemanapuram, Kadapa – 516 003, A.P. India

2. Department of Zoology, Sri Venkateswara University, Tirupati – 516 003, A.P. India

 

Abstract

Estrogenic activity is found with many phytoestrogens, including Biochanin-A (BC-A). The present study is to examine the effect of prenatal and perinatal exposure of BC-A on developmental land marks and behavior in wistar rats. The mated female rats were injected with 25, 50 and 100 mg BC-A/Kg body weight on 12th, 14th, 16th and 18th day of gestation. The exposure was continued up to completion of weaning period. After weaning, male and female pups were separated and maintained in separate cages with normal pellet diet and tap water ad libitum. The developmental landmarks and behavior of young ones were recorded starting from birth to completion of weaning. Observed significant decrease (p<0.05) in the number of live pus and sex (male/female) ratio of rat pups exposed transplacentally to BC-A when compared to control group. Body weights of young ones were measured on post natal day (PND) 1, 7, 14 and 21 and observed significant decrease (p<0.005) in 50 and 100 mg BC-A perinatally exposed young ones than the control and 25 mg BC-A exposed groups. No significant changes (p>0.05) were recorded in fur development and ear opening in exposed young ones. Whereas significant increase in pinna detachment (p<0.05), upper incisor eruption (p<0.05) and preputial separation (p<0.005) were observed in pups perinatally exposed to 100 mg BC-A only. In contrast, significant decrease in anogenital distance (AGD) (p<0.05) in male and female pups was observed at 100 mg BC-A trans-placental exposure.  In case of crown rump length (CRL), we observed significance (p<0.05) at 50 and 100 mg BC-A trans-placental exposure. Decrease in survival index was also observed on PND 1 and 14 in young rats exposed perinatally to BC-A. Besides above results, rat pups perinatally exposed to 50 and 100 mg BC-A showed significant increase in lower incisor eruption (p<0.05), eye opening (p<0.005), vaginal opening (p<0.005) and testis descent (p<0.05). These data demonstrate that significant alterations in developmental landmarks of young rats exposed transplacentally and perinatally to BC-A.

 

Keywords:  Biochanin-A, Trans-placental, Perinatal, Pups evaluation, Developmental landmarks

 

 

Ovarian intrafollicular fluid factors and their roles in follicle and ovum development in ruminants

 

Journal of Infertility and reproductive Biology, 2014, Volume 2, Issue 4, Pages 124-135, | Full Text in PDF (147 KB)

 

V. Girish Kumar1, SK Tripathi2, M. Farman1, S. Nandi2

1. College of Veterinary Sciences, KVAFSU, Bangalore campus, Hebbal, Bangalore, India

2. National Institute of Animal Nutrition and Physiology (NIANP), Bangalore, India

 

Abstract

Follicular development is controlled partly by intra-ovarian and intrafollicular factors within the ovary and partly

by  extra-ovarian  events.  Many  oocyte  derived  factors  and  granulose  cell  factors  are  also  involved  in  follicle

development. A better understanding of all the processes involved in follicular development regulated  by growth

factors  will  develop  procedures  to  enhance  the  reproductive  efficiency  in  domestic  animals.  The  present  article

discussed  the  role  of  various  growth  factors  involved  in  the  follicle  and  ovum  development,  and  biotechniques

associated with oocyte development in domestic ruminants.

 

Keywords: Growth factors, Oocyte, Follicle, Biotechniques

 

 

 

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