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J. Infertil. Reprod.
Biol. Volume 3, Issue 1, 2015 |
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Effect of isolated chromatographic fractions of Citrus
medica seeds: In vivo study on anti-implantation
and estrogenic activity in albino rats Journal of Infertility and reproductive Biology, 2015, Volume 3, Issue 1, Pages 136-144, | Full Text in PDF (782 KB)
Sharangouda J. Patil1*,
Venkatesh S2, Vishwanatha
T3, Saraswati B. Patil4 1. Department of Zoology,Government
First Grade Degree College, K. R. Puram, Bangalore,
Karnataka, India 2. Department of Biology, Vishwa
Jyothi P.U. College, Siraguppa,
Bellary, Karnataka, India 3. Department of Microbiology, Maharani’s Science College
for Women, Bangalore, Karnataka, India 4. Department of Post-Graduate Studies and Research in
Zoology, Gulbarga University, Gulbarga, Karnataka, India Abstract The therapeutic effect of medicinal plants for the treatment of various diseases is based on the presence of chemical constituents in the plants. The present investigation is aimed to justify the active principle of Citrus medica seeds by isolating chromatographic fractions of petroleum ether extract and studied for anti-implantation, pregnancy interruption and estrogenic activities in albino rats. Two fractions were obtained from thin layer chromatography (TLC) and subjected for testing to know their anti-implantation, pregnancy interruption and estrogenic activities in in vitro. Each isolated fraction (I & II) at the dose level of 50mg and 100mg/kg body weight were administered orally for 7 days in 5 groups (from day 1 to 7 of pregnancy) to pregnant rats for anti-implantation, pregnancy interruption on day 10. Also non-pregnant rats in 6 groups for estrogenic activity. Among the two isolated TLC fractions, fraction II at 50 and 100mg/kg body weight is highly significantly effective in reducing mean number of implants and exhibited 71.65 & 80.17 percent inhibition of implantation respectively. Estrogenic activity of fraction II at the dose level of 100mg/kg body weight exhibited highly significant increase in the wet weight of uterus and adrenals diameter of uterus, thickness of myometrium & endometrium and epithelial cell height. Vaginal cornification and premature opening of vagina in 6 out of 6 rats exhibited positive result and almost similar to Ethinyl estradiol administration. Furthermore, histological changes of uterus and adrenals were support the anti-implantation and estrogenic study. Hence, the results concluded that estrogenic nature of the fraction II, at 100mg/kg body weight level, possessing active constituents present in petroleum ether extract and have been proved to have significant antifertility activity in Citrus medica seeds. Keywords: Citrus medica,
Anti-implantation, Pregnancy, Estrogenic, Antifertility,
Rats Effect of environmental factors on ovarian reserve
of women living in Aral Sea area Journal of Infertility and reproductive Biology, 2015,
Volume 3, Issue 1, Pages 145-149, | Full
Text in PDF (78 KB)
Aru Balmagambetova1,
Gulmira Zhurabekova1, Ibrahim A
Abdelazim2*, Sapargali Rakhmanov1 1. Department of Normal and
Topographical Anatomy, Marat Ospanov, West
Kazakhstan State Medical University, Kazakhstan 2. Department of Obstetrics & Gynecology, Ain Shams University, Cairo, Egypt and Ahmadi Hospital,
Kuwait Oil Company (KOC), Kuwait Abstract Permanent environmental pollution by wastes arising from industries can
lead to negative effects on reproductive organs. Normal reproductive function
of women depends on the functional capacity of ovaries. This study was
designed to detect possible environmental effect on ovarian reserve of women
living in Aral Sea area. 160 women in their reproductive age were studied; 80
women from Kuwait in group I and 80 women from Shalkar,
Kazakhstan in group II. Women included in this study were evaluated using
trans-vaginal ultrasound (TVS) to detect Antral
Follicle Count (AFC) as well as Basal Ovarian Volume (BOV). AFC and BOV were
compared in both study groups. In this study, there was significant
difference between group I and group II regarding; AFC and BOV, which means
reduced ovarian reserve as indicated by AFC and BOV of women living in Aral Sea area (Kazakhstan) compared with Kuwaiti women of same age group. This study suggests possible environmental effect on ovarian reserve of women living in Aral Sea area (Kazakhstan). This study, also, concluded that, AFC
and BOV is a simple, rapid, non-invasive method for assessment of ovarian
reserve. Keywords: Environmental factors, Ovarian reserve, Aral sea area The effects of seasons on the testicular parameters and
epididymal sperm of the Iranian river buffalo (Bubalus bubalis) Journal of Infertility and reproductive Biology, 2015,
Volume 3, Issue 1, Pages 150-154, | Full
Text in PDF (1.26 MB) Saeed
Moosa-Ali1, Farid Barati1*, Saleh Esmaeilzadeh2, Dariush
Gharibi2, Mahmood Khaksary
Mahabady3 1. Department of Clinical Science, Faculty of Veterinary
Medicine, Shahid Chamran University, Ahvaz, Iran 2. Department of pathobiological sciences, Faculty of
Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran 3. Department of Basic Sciences, Faculty of
Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran Abstract The aim of the present study
was to evaluate the effects of different seasons on the morphometric
testicular parameters and the respective epididymal
sperm parameters. Total numbers of 104 scrota of pubertal buffalo bull (24-48
months) include their contents were provided from the local slaughterhouse.
Scrotal circumference was measured on its place before dissection.
Morphological parameters of testis and epididymis
and epididymal sperm were analyzed. The results
showed the highest scrotal circumference and testicular weight as well as
retrieved sperm concentrations in spring. However, sperm tail and midpiece abnormalities were highest and the sperm progressive
motility was the lowest during spring. During summer the lowest testicular
weight and retrieved sperm concentration as well as low sperm progressive
motility was considered. In conclusion, the results of the present study
confirm direct influence of different seasons on the epididymal
sperm and the testicular parameters of the Iranian river buffalo bull. Keywords: Season, Iranian river
buffalo, Epididyme, Sperm, Testicular morphometry |
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Spinal cord histomorphometric alterations in the male and female
diabetic Sprague-Dawley rats Journal of Infertility and reproductive Biology, 2015,
Volume 3, Issue 1, Pages 155-159, |
Full
Text in PDF (218 KB) Hooman
Hematian Department of Veterinary sciences, Yasooj Branch, Islamic Azad University, Yasooj, Iran Abstract This study was conducted to
evaluate the effects ofdiabetes on spinal cord
structure in male and female rats and to compare the alterations of the spinal cord in both genders.Twenty adult female and the same number of male
Sprague Dawley rats were divided in four groups, aseach group
contained 5 males and 5 females. Diabetes was induced in 3 groups by alloxan agent. All of the animals were kept at the same
condition in animal house but each group was kept for different periods of
time. Afterexperiment, animals were anaesthetized
and scarified. Then the spinal cord
was collected from
all rats. Various histological parameters
were determined using
histological techniques. Results revealed that the diameters andthe cell numbers of the gray matter and the white matter have decreased in
all test groups;
however these alterations
have been greater
and more significant
in the males
than females. Diabetes exhibit deleterious effects on male and female
spinal cords, although these effects are more serious in males. Keywords: Diabetes, Rat, Gender, Alloxan, Spinal cord Clinical diagnosis and
management of Swyer syndromeexhibiting
different genetic variants: Current review of the literature Journal of Infertility and reproductive Biology, 2015,
Volume 3, Issue 1, Pages 160-164, |
Full
Text in PDF (66 KB)
Serkan Kahyaoglu Department of Reproductive
Endocrinology, Zekai Tahir
Burak Women’s Health Education and Research
Hospital, Ankara, Turkey Abstract All genetical
causes of pure
gonadal
dygenesis,
Swyer
syndrome, have not
been discovered yet.
Clinical and molecular
studies are needed
to identify novel
genetic alterations that
result in this
type of sexual
differentiation disorder. Swyer syndrome is
a rare form of gonadal dysgenesis
syndromes characterized with a 46, XY karyotype. A
non-functional Y chromosome caused by various genetic mutations results in a
deficient testis determining pathway and cessation of
AMH and androgen
secretion. As a
result, normally developed
mullerian
structures (upper two thirds of vagina, cervix, uterus and
fallopian tubes) stand by nonmasculinized internal
and external genitalia. Several genes are involved in the process of sex
differentiation, including SRY, RSPO1, SOX9, NR5A1, WT1, NR0B1 and WNT4. Among
genetic mutations resulting with
a deficient testis determining
pathway, for 10-15%
of patients a mutation that
inactivates the SRY gene located on the long arm of Y chromosome is the ethiological factor. Defect in a different testis
determining factor (TDF) such asthe SF-1 gene is
proposed as another etiological factor resulting with Swyer
syndrome. The absence of known mutationsin the SRY
gene indicates that molecular defects could be present in the untranslated
regulatory regions of the SRY gene or presence of other deficient gene(s) could explain the
disorder. A phenotypically female patient
presenting with primary amenorrhea, delayed puberty, normal internal
genitalia except streak
gonads and hypergonadotropic hyponadism should
be evaluated by
advanced genetic investigations
to discover novel mutations for puregonadal dysgenesis. Keywords: Swyer syndrome,
Amenorrhea, Gonadoblastoma, Streak gonad, Disorders
of sex development, Y chromosome Journal of
Infertility and Reproductive Biology Copyright 2013,
All Rights Reserved Dorma Trading, Est.
Publishing Manager Address:
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