J. Infertil. Reprod. Biol.

Volume 3, Issue 4, 2015


Association study of Yq11 AZFc subdeletion and recurrent pregnancy loss-A study in South Indian males


Journal of Infertility and reproductive Biology, 2015, Volume 3, Issue 4, Pages 226-233, | Full Text in PDF (113 KB)


Kavitha Papanna, Shivaprasad H Satyanarayan, Vineeth V Satidevi, Suttur. S Malini

Molecular Reproductive and Human Genetics Laboratory, Department of Studies in Zoology, Manasagangotri, Mysore, Karnataka, India




The etiologies in recurrent pregnancy loss were focused only towards females with less consideration of male factors. This study was undertaken to analyze the association of AZF deletions in idiopathic recurrent pregnancy loss among South Indian couples. Eighty males whose female partners with the history of two or more idiopathic recurrent pregnancy loss were considered as a case group and fifty male volunteers with proven fertility as a control group. Semen and blood samples were collected from the entire study subject and examined for functionality of the sperm along with routine semen analysis. Standard PCR was performed for Yq microdeletion analysis in AZFa, b and c regions along with AZFc subdeletion analysis. RPL group showed statistically significant lower scores for total motility and sperm function test than the control. Abnormal semen parameters were reported in 18.8% among RPL males. AZFc subdeletions were reported in 8.75% of RPL males, none of the RPL individuals including control group showed no deletion for AZFa, AZFb and AZFc. We observed subnormal sperm function scores in AZFc sub deleted subjects. No significant association was observed between routine semen parameters and AZFc subdeletions. Our results revealed the potential association of abnormal sperm functioning and AZFc subdeletion in the idiopathic RPL cases in South Indian population.


Keywords: Recurrent pregnancy loss, Sperm function test, Y chromosome, AZF microdeletions, AZFc subdeletions


Ectopic pregnancy in tubal stump after ipsilaterial salpigo-oohorectomy: an unusual and rare case report


Journal of Infertility and reproductive Biology, 2015, Volume 3, Issue 4, Pages 234-236, | Full Text in PDF (190 KB)


Ranjan Agrawal* , Parbodh Kumar

Department of Pathology, Rohilkhand Medical College & Hospital, Bareilly (UP); India




A 35-years-old female presented with severe abdominal pain, vomiting and spotting per vaginum since one day following 2 months amenorrhoea. Ultrasound abdomen revealed right sided tubal pregnancy. She gave a history of right sided salpingo-oophorectomy 15 years back. The case is unusual due to the ectopic occurring on the same side of salpingectomy and also since the ectopic occurred after such a long interval.


Keywords: Ipsilateral, Ectopic pregnancy, Tubal stump, Salpingo-oophorectomy


Methyl farnesoate induced ovarian vitellogenin syntehsis in freshwater rice field crab Oziothelphusa senex senex Fabricious


Journal of Infertility and reproductive Biology, 2015, Volume 3, Issue 4, Pages 237-244, | Full Text in PDF (176 KB)


B. Srinivasa Reddy, P. Ramachandra Reddy

Department of Biochemistry, Yogi Vemana University, Vemanapuram, Kadapa 516 003, A.P. INDIA



Reproduction in crustaceans is under the control of an array of hormones, internal and external factors including the terpenoid hormone methyl farneosate (MF). In the present study an attempt was made to induce vitellogenesis in crab Oziotelphusa senex senex by injecting MF on every alternative day up to 28 days at a concentration of 10-8 moles/crab. The concurrent control group received vehicle on par with MF injected group and eyestalk ablated group as positive control were maintained. The crabs were sacrificed on 7th, 14th, 21st and 28th day of experiment to check the stage of reproduction and to measure the ovarian vitellogenin levels. Significant (p<0.001) increase was observed in ovarian vitellogenin levels in MF injected and eye stalk ablation groups on 7th, 14th, 21st and 28thday of experiment, when compared to concurrent controls which are in immature stage throughout the experimentation. Also observed increase in the ovarian maturation and ovarian vitellogenin levels are significant in MF injected group than the eyestalk ablated group. Observed two reproductive cycles in the MF injected group of with a mean days of 112 in 28 days experiment. Whereas positive control group (ESX) is about to complete one reproductive cycle by the end of the experiment. The results of the present study clear indicate that MF induces vitellogenin synthesis there by ovarian maturation in crab by direct and/or indirect action.


Keywords: Crab, Ovarian maturation, Vitellogenin, Eyestalk ablation, Methyl farnesoate



Early clomiphene citrate for ovulation induction in polycystic ovary syndrome



Journal of Infertility and reproductive Biology, 2015, Volume 3, Issue 4, Pages 245-249, | Full Text in PDF (78 KB)


Mohamed Hussain1, Amr H. Yehia1, Ibrahim A. Abdelazim1,2, Assem A. M. Elbiaa1, Khaleed M. Abdelrazak1, Mohamed M. Farghali1

1. Department of Obstetrics and Gynaecology, Ain Shams University, Cairo, Egypt.

2. Department of Obstetrics and Gynaecology, Ahmadi Hospital, Kuwait Oil Company (KOC), Kuwait.




This study was designed to evaluate effect of starting Clomiphene Citrate (CC) in oligomenorrheic women with PCOS immediately after progestin therapy without waiting withdrawal bleeding versus CC on day 2 of the withdrawal bleeding. Eighty Four (84) women with PCOS, oligo/amenorrhea were studied. Studied women received oral progestin to induce withdrawal bleeding. Women who started CC immediately after oral progestin without waiting withdrawal bleeding were included in group I and women who started CC at the same dose and for the same duration, but on day 2 of the induced withdrawal bleeding were included in group II. Transvaginal ultrasound scan (TVS) for folliculometry and endometrial thickness was performed after end of CC treatment and repeated every 48 hours till mature follicle (s) was detected. Results showed that rate of detection of one mature follicle was slightly higher in early CC group compared with late CC group 25 (59.5%) versus 21 (50%); respectively), but this difference was not significant. Endometrial thickness at detection of mature follicle was significantly higher in early CC group compared with late CC group (10.42 1.93 mm versus 8.7 1.77; respectively), clinical pregnancy rate was slightly higher in early CC group compared with late CC group (6(14.3%) versus 4(9.5%); respectively); but this difference was not significant. It was concluded that early CC administration for ovulation induction in PCOS seems to be associated with significantly thicker endometrium, slightly higher ovulation and pregnancy rates, compared to the conventional CC administration.


Keywords: Clomiphene citrate, Ovulation induction, Polycystic ovary syndrome



Influence of estrogen, progesterone and their synthetic derivatives on ovarian functions



Journal of Infertility and reproductive Biology, 2015, Volume 3, Issue 4, Pages 250-254, | Full Text in PDF (76 KB)


Mohammed Farman1*, Shiv Kumar Tripathi2, Deepthi K Babu3, Sumanta Nandi 2, V Girish Kumar1


1. Department of Veterinary Biochemistry, Veterinary College, Bangalore-560024, India

2. National Institute of Animal Nutrition and Physiology, Bangalore-560030, India

3. Sathyabama University Chennai-600119, India.



Estrogen and progesterone, the main sex steroids mostly bound to plasma proteins while circulating in the bloodstream. Only unbound estrogen and progesterone are biologically active. Gonadotrophins like Luteinizing hormone (LH) and Follicle stimulating hormone (FSH) promote ovulation and stimulate secretion of the sex hormones-estrogen and progesterone from the ovaries. These sex steroids stimulate the target organs of the reproductive system and inhibit as well as around the time of ovulation stimulate gonadotropin secretion. Since estrogen circulating in the blood can negatively feed-back to reduce circulating levels of FSH and LH, most oral contraceptives contain a synthetic estrogen, along with a progestin. The present article overviews the role of estrogen, progesterone and their synthetic derivatives on ovarian functions.


Keywords: Estrogen, Progesterone, Progestin, Ovary


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