J. Infertil. Reprod. Biol.
Volume 4, Issue 1, 2016
Relation between Aromatase Gene CYP19 Variation and Hyperandrogenism in Polycystic Ovary Syndrome Egyptian Women
Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 1, Pages 1-5, | Full Text in PDF (68 KB)
Mostafa R1, Mohammed M. Al-Sherbeeny1, Ibrahim A. Abdelazim1,2, Ahmed A. Fahmy1, Mohamed M. Farghali1, Abdel-Fatah M1, Mahran M3
1. Department of Obstetrics and Gynaecology, Ain-Shams University, Cairo, Egypt
2. Department of Obstetrics and Gynaecology, Ahmadi Hospital, Kuwait Oil Company (KOC), Kuwait
3. Department of Clinical Pathology, Ain-Shams University, Cairo, Egypt
This study designed to detect the relation between the aromatase CYP19 gene SNP50 variation and hyperandrogenism in polycystic ovary syndrome (PCOS) Egyptian women. Sixty women included in this comparative study and divided into 2 groups; 30 PCOS women with clinical hyperandrogenism included in study group and 30 healthy non-PCOS women in control group. PCOS diagnosis based on the Rotterdam criteria. Studied women underwent complete physical examination with calculation of body mass index (BMI) and assessment of hirsutism. Studied women subjected to hormonal profile and to CYP19 genotyping by collecting 5 ml whole blood on EDITA for DNA extraction and SNP 50 (rs2414096) genotype to detect the relation between the aromatase CYP19 gene SNP50 variation and hyperandrogenism in polycystic ovary syndrome Egyptian women. Results showed that LH and LH/FSH ratio were significantly high in PCOS women (16.7 ± 2.1 IU/l and 2.01 ± 3.3; respectively) compared to controls (3.5 ± 1.3 IU/l and 0.45 ± 2.1; respectively), also, serum testosterone and estradiol were significantly high in PCOS women (3.2 ± 3.8 nMol/l and 245.5 ± 198 pMol/l; respectively) compared to controls (1.2 ± 2.7 nMol/l and 176.2 ± 142.3 pMol/l; respectively). CYP19 rs2424096 genotypic distribution AG alleles was significantly high in PCOS women compared to controls (11 (44%) versus 2 (8.4%); respectively) (p = 0.02). Estradiol/testosterone ratio was significantly low in AG genotypes PCOS compared to AG genotype controls (80.8 ± 7.3 versus 162.4 ± 6.8) (P = 0.001). In conclusion, CYP 19 rs2414096 polymorphism is associated with aromatase deficiency or reduced aromatase activity with subsequent hyperandrogenism in PCOS Egyptian women.
Keywords: Subdeletions Aromatase Gene, Hyperandrogenism, PCOS, Egyptian
Effect of sperm motility enhancers on in vitro fertilization and embryo development of buffalo oocytes
Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 1, Pages 6-10, | Full Text in PDF (150 KB)
Eman Mahmoud Abu-El Naga1, Hossam El-Sheikh Ali2, Magdy Ramdan Badr3, Ashraf Mohamed El Desouky2, and Samy Moawad Zaabel2
1. Theriogenology Department, Faculty of Veterinary Medicine, Aswan University, Egypt
2. Theriogenology Department, Faculty of Veterinary Medicine, Mansoura University, Egypt
3. Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Al Haram, Giza, Egypt
The present study was carried out to investigate the effect of adding various concentrations of penicillaminen, hypotaurine and epinephrine (PHE) to the in vitro fertilization (IVF) media on the fertilization rate and embryonic development up to the blastocyst stage of buffalo oocytes. In vitro matured oocytes were fertilized in different PHE concentration (10, 40 and 80 µM/ml) in the presence of 5.0 mM caffeine. After IVF, the fertilization rate, the cleavage rate and the development rates up to the blastocyst stage were assessed. The present results revealed that supplementation of the fertilization media with 40 mg/ml PHE to the fertilization media increased significantly (P<0.05) the fertilization, cleavage rates and the development rates up to the morula and blastocyst stage (73.15±2.19, 59.90±6.19, 48.60±24.88 and 33.13±7.04%, respectively) compared to those of the control groups (38.83±4.82, 37.13±2.43, 7.75±1.937.75±1.93 and 2.50±1.04%, respectively). In conclusion, the current results demonstrated that addition of 40 µM/ml PHE to fertilization medium, had a positive effect on buffalo oocytes, fertilization and supported embryonic development up to the blastocyst stage.
Keywords: In vitro fertilization, oocyte, embryo development, buffalo, PHE
Prevalence of Mitochondrial DNA Nucleotide Substitution Mutations in Male Infertile Cases of Northeast India
Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 1, Pages 11-21, | Full Text in PDF (127 KB)
Purnali Nath Barbhuiya1, Anannya Gogoi2, Giasuddin Ahmed3, Rita Mahanta4
1. Principal Investigator, WOS-A Project, Cotton College, Guwahati, Assam-781001, India and Research Scholar, Dept. of Biotechnology, Gauhati University, Guwahati, Assam-781014, India
2. DBT-Sponsored Institutional Biotech Hubs, Cotton College, Guwahati, Assam-781001, India
3. Department of Biotechnology, Gauhati University, Guwahati, Assam-781014, India
4. Department of Zoology, Cotton College, Guwahati, Assam-781001, India
In the present study a systematic sequence analysis of 6 mitochondrial genes (NADH dehydrogenase 2, NADH dehydrogenase 3, NADH dehydrogenase 4L, NADH dehydrogenase 4, Adenosine triphosphate synthase 6 and Adenosine triphosphate synthase 8) was carried out in 50 clinically diagnosed infertile cases of various types and severity of Northeast Indian population. A total of 50 different nucleotide substitutions were detected of which 24 are new. Mutations like A4824G, G10373A and A9156G in azoospermic males, G5054A, G5046A, C10268T, C8930T, T9148C and T9128C in oligozoospermic and G8998A and G8557C in cryotozoospermic individuals are observed for first time in this study. Most of the infertile men are found to belong from haplogroup Y.
Keywords: Male infertility, Mitochondrial DNA, Single nucleotide polymorphism
Role of vertebrate steroids in the regulation of testicular development in the fresh water crab, Oziotelphusa senex senex
Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 1, Pages 22-27, | Full Text in PDF (187 KB)
Modugapalem. Hemalatha1, Kasa. Parameswari1, Battini. Kishori1, Pamanji. Sreenivasula Reddy2
1. Department of Obstetrics and Gynaecology, Ain Shams University, Cairo, Egypt.
2. Department of Obstetrics and Gynaecology, Ahmadi Hospital, Kuwait Oil Company (KOC), Kuwait.
This study was designed to evaluate effect of starting Clomiphene Citrate (CC) in oligomenorrheic women with PCOS immediately after progestin therapy without waiting withdrawal bleeding versus CC on day 2 of the withdrawal bleeding. Eighty Four (84) women with PCOS, oligo/amenorrhea were studied. Studied women received oral progestin to induce withdrawal bleeding. Women who started CC immediately after oral progestin without waiting withdrawal bleeding were included in group I and women who started CC at the same dose and for the same duration, but on day 2 of the induced withdrawal bleeding were included in group II. Transvaginal ultrasound scan (TVS) for folliculometry and endometrial thickness was performed after end of CC treatment and repeated every 48 hours till mature follicle (s) was detected. Results showed that rate of detection of one mature follicle was slightly higher in early CC group compared with late CC group 25 (59.5%) versus 21 (50%); respectively), but this difference was not significant. Endometrial thickness at detection of mature follicle was significantly higher in early CC group compared with late CC group (10.42 ± 1.93 mm versus 8.7 ± 1.77; respectively), clinical pregnancy rate was slightly higher in early CC group compared with late CC group (6(14.3%) versus 4(9.5%); respectively); but this difference was not significant. It was concluded that early CC administration for ovulation induction in PCOS seems to be associated with significantly thicker endometrium, slightly higher ovulation and pregnancy rates, compared to the conventional CC administration.
Keywords: Clomiphene citrate, Ovulation induction, Polycystic ovary syndrome
Pre and Postnatal Exposure of Baicalein (Flavonoid) on Developmental Landmarks of Mice
Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 1, Pages 28-34, | Full Text in PDF (288 KB)
Vaadala Sridevi, Mythatha Grace Sugandha Sowjanya, Pamuru Ramachandra Reddy
Department of Biochemistry, Yogi Vemana University, Vemanapuram, Kadapa – 516 003, A.P. INDIA
Baicalein a flavonoid comes under phytoestrogen family found to have estrogenic activity in mammals. The present investigation aimed to study the effect of baicalein on behaviour and developmental land marks of Wistar mice. Baicalein at a dose of 30, 60 and 90 mg/Kg body weight were injected to three different groups of pregnant female mice on gestation days 11, 13, 15 and 17, whereas controls were injected with vehicle. Controls and experimental groups were allowed to deliver pups and baicalein exposure was continued during lactation. No clinical signs of toxicity were observed in the behaviour of experimental animals during experimentation when compared to controls. Observed significant decrease (P<0.05) in number of live pups in baicalein received females when compared to controls. Also observed decrease in the survival index of pups on postnatal day 4 and 21 in experimental groups. Significant (P<0.05) decrease in early onset of puberty in females (vaginal opening) and delay in testes descent in males of experimental groups were also observed. Also observed significant decrease (P<0.05) in the body weight of pups exposed perinatally to baicalein on postnatal day (PND) 1, 7, 14 and 21, along with significant decrease (P<0.05) in crown rump length when compared to controls. No significant change was observed in pinna detachment, eye opening and fur development in experimental groups when compared to controls. It is clear from the results that baicalein interferes in developmental landmarks in mice.
Keywords: Baicalein, Perinatal, Developmental landmarks, Puberty, Testes descent
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