J. Infertil. Reprod. Biol.
Volume 4, Issue 2, 2016
Semen quality and age-dependent changes among male participants with normal sperm count in Qom, Iran
Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 2, Pages 35-39, | Full Text in PDF (267 KB)|
Mohsen Sheykhhasan, Mahdieh Ghiasi
Laboratory of Stem Cell, The Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran
Background: One of the most conventional causes of male infertility is increasing age, which has significant effects on sperm physiology. In advanced ages, degenerative alterations in germinal epithelium, decreased number of Leydig cells, and their functions affect spermatogenesis through a decrease in testosterone level. So it is important to know whether advanced paternal age is associated with decreased semen quality.
Methods: Semen samples of 144 men were collected and semen parameters (volume, concentration, and normal morphology) were evaluated after semen liquefaction at room temperature. Statistical analyzes of data was done using SPSS software.
Results: we showed that increased total sperm count was correlated with increasing age (P≤0. 05). Analyzing the percentage of normal morphology showed a significant difference between group I and III (p≤0. 05) while there was no significant difference in comparison of other groups. In terms of the semen volume, no significant difference was shown.
Conclusion: We showed that with increasing age sperm concentration significantly increases. It may be possible that spermatogenesis could be abnormally accelerated because of an impaired responsiveness of the testes to endocrinological influences. Moreover, we obtained results in terms of normal morphology percentage pursuant to previous studies, while in older group this parameter was lower than the youngest group significantly. The present study predicted that aging negatively affect the morphology of sperms.
Keywords: Male, Aging, Sperm count, Morphology
Efficacy of zeta potential as an effective sperm selection method for ICSI in smokers and obese patients
Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 2, Pages 40-44, | Full Text in PDF (60 KB)|
Sreejaya Pazhedath, Nirmala Periyaswamy
Nehru Arts and Science College, Coimbatore, India
Background: Life style factors and oxidative stress are two key factors which cause male infertility. This work was aimed to study the efficacy of Zeta potential method as a reliable sperm selection technique for ICSI in smokers and obese patients, whose sperms in which oxidative stress and DNA damages are expected to be high.
Methods: This is a prospective study performed in 50 smokers and 50 obese patients and compared the zeta potential vs. normal swim down sperm selection method to assess the motility and DNA fragmentation.
Results: In smokers, the percentage of motility with swim down was 72.87±4.65 while in zeta it was 76.92±4.69. The DNA fragmentation in swim down vs. zeta was 76.24±6.91 and 67.35±6.05 respectively. In non smokers the percentage of motility with swim down vs. zeta yielded 76.02±6.93 and 78.74±7.4 respectively. The percentage of DNA fragmentation was 74.52±6.4 and 67.99±6.04. In obese patients swim down method yielded percentage of motility with 74.12±4.67 and percentage of DNA fragmentation with 74.52±6.4. The zeta potential yielded 77.10±5.67 percentage of motility and 67.99±6.04 percentage of DNA fragmentation. Student’s t-test showed significant difference between both methods in motility and DNA fragmentation percentage with p-value less than 0.05.
Conclusion: Zeta potential method is an effective sperm selection technique in male infertile patients especially in smokers and obese patients whose body contains high reactive oxygen species level. By comparing to other methods of sperm selection, the zeta potential method is easy to perform, less expensive and can be carried out in any basic andrology lab.
Keywords: ICSI, Zeta potential, ROS, Motility, DNA fragmentation
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