J. Infertil. Reprod. Biol.

Volume 4, Issue 2, 2016


Semen quality and age-dependent changes among male participants with normal sperm count in Qom, Iran


Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 2, Pages 35-39, | Full Text in PDF (267 KB)|

Mohsen Sheykhhasan, Mahdieh Ghiasi


Laboratory of Stem Cell, The Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran



Background: One of the most conventional causes of male infertility is increasing age, which has significant effects on sperm physiology. In advanced ages, degenerative alterations in germinal epithelium, decreased number of Leydig cells, and their functions affect spermatogenesis through a decrease in testosterone level. So it is important to know whether advanced paternal age is associated with decreased semen quality.

Methods: Semen samples of 144 men were collected and semen parameters (volume, concentration, and normal morphology) were evaluated after semen liquefaction at room temperature. Statistical analyzes of data was done using SPSS software.

Results: we showed that increased total sperm count was correlated with increasing age (P≤0. 05). Analyzing the percentage of normal morphology showed a significant difference between group I and III (p≤0. 05) while there was no significant difference in comparison of other groups. In terms of the semen volume, no significant difference was shown.

Conclusion: We showed that with increasing age sperm concentration significantly increases. It may be possible that spermatogenesis could be abnormally accelerated because of an impaired responsiveness of the testes to endocrinological influences. Moreover, we obtained results in terms of normal morphology percentage pursuant to previous studies, while in older group this parameter was lower than the youngest group significantly. The present study predicted that aging negatively affect the morphology of sperms.


Keywords: Male,  Aging, Sperm count, Morphology



Efficacy of zeta potential as an effective sperm selection method for ICSI in smokers and obese patients


Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 2, Pages 40-44, | Full Text in PDF (60 KB)|

Sreejaya Pazhedath, Nirmala Periyaswamy


Nehru Arts and Science College, Coimbatore, India



Background: Life style factors and oxidative stress are two key factors which cause male infertility. This work was aimed to study the efficacy of Zeta potential method as a reliable sperm selection technique for ICSI in smokers and obese patients, whose sperms in which oxidative stress and DNA damages are expected to be high.

Methods: This is a prospective study performed in 50 smokers and 50 obese patients and compared the zeta potential vs. normal swim down sperm selection method to assess the motility and DNA fragmentation.

Results: In smokers, the percentage of motility with swim down was 72.87±4.65 while in zeta it was 76.92±4.69. The DNA fragmentation in swim down vs. zeta was 76.24±6.91 and 67.35±6.05 respectively. In non smokers the percentage of motility with swim down vs. zeta yielded 76.02±6.93 and 78.74±7.4 respectively. The percentage of DNA fragmentation was 74.52±6.4 and 67.99±6.04. In obese patients swim down method yielded percentage of motility with 74.12±4.67 and percentage of DNA fragmentation with 74.52±6.4. The zeta potential yielded 77.10±5.67 percentage of motility and 67.99±6.04 percentage of DNA fragmentation. Student’s t-test showed significant difference between both methods in motility and DNA fragmentation percentage with p-value less than 0.05.

Conclusion: Zeta potential method is an effective sperm selection technique in male infertile patients especially in smokers and obese patients whose body contains high reactive oxygen species level. By comparing to other methods of sperm selection, the zeta potential method is easy to perform, less expensive and can be carried out in any basic andrology lab.


Keywords: ICSI, Zeta potential, ROS, Motility, DNA fragmentation



Effects of Phoenix dactylifera on the testes, epididymal sperm pattern and hormonal profiles of male Wistar rats


Journal of Infertility and reproductive Biology, 2016, Volume 4, Issue 2, Pages 45-50, | Full Text in PDF (337 KB)|

Nathan Isaac Dibal1, Joseph Olajide Hambolu2, Adebayo Adekunle Buraimoh3


1. Department of Human Anatomy, University of Maiduguri, Borno State, Nigeria

2. Department of Veterinary Anatomy, Ahmadu Bello University Zaria, Kaduna State, Nigeria

3. Department of Human Anatomy, Kaduna State University, Kaduna, Nigeria



Background: Phoenix dactylifera belongs to the family Arecaceae, It is rich in vitamins, steroids, flavonoid, saponins and simple sugars. The aim of the study is to evaluate the effect of date palm fruit on testes, epididymal sperm pattern and hormonal profiles of male Wistar rats.

Methods: Twenty (20) male Wistar Rats were divided into four groups of five rats each. Three (3) experimental groups were treated with the extract at 250 mg/kg, 500 mg/kg, and 1000 mg/kg body weight for 35 days while the control receives distilled water. The rats were sacrificed on the 36th day and the testes and epididymis were dissected and weighed, the testes were processed for light microscopic study; blood samples were collected for hormonal assay [Testosterone, Follicle Stimulating Hormone and Luteinizing Hormone]. Sperm samples from the epididymis were collected and counted; a smear was made and stained with cresyl violent to determine sperm morphology. Morphometric analysis was performed to measure seminiferous tubular diameter, size of interstices and epididymal epithelial thickness.

Results: The result showed degeneration of spermatogenic cells, destruction of seminiferous tubular membranes and leydig cells with significant decrease in serum testosterone levels but no significant change in FSH and LH levels. There was also significant decrease in sperm count, motility and morphology with decrease in size of interstices at P≤0.05.

Conclusion: In conclusion, the components of the extract have the potentials of causing infertility in male Wistar Rats by decreasing serum testosterone levels, sperm count, sperm motility and sperm morphology.


Keywords: Phoenix dactylifera, Leydig cells, Testes, Testosterone, Sperm count, Epididymis


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