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J. Infertil. Reprod. Biol.

Volume 5, Issue 3, 2017

 

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Subjection to Cadmium Chloride Compromises Oocyte Maturation, Fertilization Competence and Subsequent Embryo Development in Mature Female Mice

 

Journal of Infertility and reproductive Biology, 2017, Volume 5, Issue 3, Pages 12-14, | Full Text in PDF | Full Text in HTML

 

Sanaz Alaee1, Zahra Khodabandeh2, Raheleh Asadollahpour3

 

1. Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

2. Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

3. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran

 

Abstract

Background: Cadmium is one of the main trace elements in the environment which in high doses could have adverse effects on human health, including male and female reproduction potential. Therefore, the present study was carried out to determine the possible toxic effect of this trace element on oocyte maturation.

Materials and Methods: Mature female NMRI mice (68 weeks old) were divided into three groups and received 0 (control), 1 mg/kg and 2 mg/kg cadmium chloride intraperitoneally for five days. Then all mice received PMSG and after 48 hours received hCG intraperitoneally. 14 hours later, cumulus-oocyte complexes were extracted from oviducts and the percentage of degenerated, germinal vesicle, metaphase I and metaphase II was recorded. Also, after performing in vitro fertilization, the cleavage and blastocyst formation rate was assessed.

Results: The degeneration and maturation rate of oocytes was significantly higher and lower, respectively, in groups treated with different concentrations of cadmium compared with the control group (p<0.05). The number of fertilized, cleaved and blastocysts was significantly higher in groups that received cadmium compared with the control group (p<0.05).

Conclusion: Even low dose and short duration exposure to cadmium has obvious detrimental effect on oocyte maturation, fertilization competence and subsequent embryo development.

 

Keywords: Cadmium, Oocytes, Fertilization, Mice

 

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