J. Infertil. Reprod. Biol.

Volume 6, Issue 4, 2018


A Timeline Morphological Study of TNF-α Induced Changes in Mice Oocytes


Journal of Infertility and reproductive Biology, 2018, Volume 6, Issue 4, Pages 16-18, | Full Text in PDF and HTML  

Ranjana Rana, Mona Sharma

Department of Reproductive Biology, AIIMS, New Delhi, India


Abstract: Inflammatory cytokines induce cellular degenerative and apoptotic changes. Studies have shown increase in cytokine levels in patients with premature ovarian aging or failure (POF) where excessive oocyte atresia is the major pathogenic feature. Enhanced expression of tumour necrosis factor-alpha (TNF-α), a pro-inflammatory cytokine, and its receptors have been found in the oocytes of immunized mice with experimental immune ovarian failure. The aim of the present work was to study the direct effects of TNF-α on naked oocytes. Oocytes were isolated from superovulated Swiss Albino female mice and treated with different concentrations of TNF-α (0.1, 1, 10 and 100ng/ml). Morphological scoring was assessed based on shape, cytoplasmic and extra-cytoplasmic features of the oocytes. It was found that TNF-α could induce morphological degenerative changes in mice oocytes at all the concentrations, maximum being at 10 ng/ml, suggesting a direct effect of this inflammatory cytokine on mammalian oocytes. Present study was a small piece of work that could highlight the possible direct effects of TNF-α on mice oocytes. Similar larger studies need to be planned in near future for validation. Further studies should also be planned in animal models of immune POF or autoimmune ovarian disorders to see the effect of anti-TNF-α treatment on ovarian physiology. Our lab is planning to continue the study further to confirm the morphological features as apoptotic by TUNEL or caspase staining and to find out the possible preventive or therapeutic options.


Keywords: Cytokines, Tumor necrosis factor-alpha, Oocytes



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