J. Infertil. Reprod. Biol.
Volume 7, Issue 2, 2019
Spermatogenic and Steroidogenesis Functions of Rat Testis following Exposure to Alafia barteri Leaf Extracts
Adelakun Sunday Aderemi1*, Ogunlade Babatunde Samson1, Aniah J. Akomaye2, Ibiayo Ayooluwa Gabriel3
1 Department of Human Anatomy, School of Health and Health Technology, Federal University of Technology, Akure, Nigeria.
2 Department of Anatomy, College of Medicine, University of Abuja, Federal Capital Territory (FCT), Nigeria.
3 Department of Anatomy, College of Medicine, University of Lagos, Lagos, Nigeria
Abstract: Background: In Africa and other continents herbal remedies have played essential roles in the treatment of all kinds of diseases since early times. Objectives: To study the impact of Alafia barteri on spermatogenesis and steroidogenesis in rats. Materials and Methods: Twenty (n=20) adult male wistar rats randomly assigned into four groups of five rats each consisting of a control which received only 2 ml/kg normal saline and treatment groups at the doses of 100, 300 and 500 mg/kg body weight daily for 28 days via gastric gavage. Parameter tested includes sperm quality, reproductive hormone, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and malondialdehyde (MDA) levels. Results: Sperm quality, total serum testosterone, SOD, CAT, and GPX levels were significantly increased in the Alafia barteri group in comparison to controls (P < 0.05). Also, rats in the Alafia barteri group showed a significant decreased in the level of plasma MDA (P < 0.05) in comparison to controls. There was a decreased in Follicle Stimulating Hormone (FSH) but no significant increase in the levels of leutinizing Hormone (LH). Conclusions: The administration of Alafia barteri extract significantly increased the sperm quality and possesses profertility properties, these profertility properties can be exploited in male fertility therapy.
Keywords: Alafia barteri, Catalas, Spermatogenesis, Superoxide Dismutase, Testosterone
Stringency in Polycystic Ovary Syndrome (PCOS) Criteria and Application in Clinical Study
Catherine Schneider1, Heather Huddleston2, Umesh Masharani2, Ashley Mason2, Lynda Frassetto2
1U California Berkeley,
2U California San Francisco
Abstract: Background: In this manuscript, we review the various criteria used to diagnosis PCOS, and discuss how the specific diagnostic criteria used can impact recruitment for PCOS studies. Context: PCOS is a common diagnosis, but with a number of differing definitions. We were interested in addressing these differing stringencies and application in clinical trial, such as our group’s PCOS diet study. Methods: For our study on the effects of diets to alter insulin resistance, we adopted the one using more stringent criteria, consisting of biochemical abnormalities, menstrual abnormalities, insulin resistance and abnormal ovarian size and structure. Our study actively recruits from PCOS clinics in the Bay Area. We reported number of women successfully recruited using our PCOS diagnosis stringency, and how these numbers differ from women referred to PCOS clinics in the Bay Area. We also report the reasons patients did not fit our diagnosis criteria to shed insight into how diagnosis differ between healthcare professionals. Results: To our surprise, the vast majority of subjects seen in the tertiary referral PCOS center at UCSF did not qualify for the study. Conclusion: The definition of PCOS may be important in study design, and can impact the ability to recruit for the study.
Keywords: Insulin resistance, obesity, clinical trial, diet, menstrual cycle, hyperandrogenism, polycystic ovary syndrome
Journal of Infertility and Reproductive Biology
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